ETOPIC APPLICATION OF LIQUID DISINFECTANT IN THE TREATMENT OF WOUND USING ANIMAL MODEL (ALBINO RATS)

ABSTRACT

 The aim of this topic was to determine the etopic application of disinfectants on wounds using animal model (Albino rats), the objective of this topic was to determine the time duration of healing on these animal models, the efficacy of the chosen disinfectants on identified isolates from the wounds. The specimen wound were swabbed, cultured on nutrient agar and incubated at 370C for 24 hours. The pure culture of the organisms was gram stained which reliably indicates sterile and mixed abscesses as well as those containing pure Staphylococcus aureus. The time duration of healing between the test control and the disinfectants showed that the test control healed faster but was scarred while the disinfectant healed completely within a period of seven (7) to ten (10) days. The efficacy of the chosen disinfectants which were Dettol (a make of Reckitt Benckiser., man date: 04/18, exp date: 04/21), izal ( a make of Nath Peters Hygeian Ltd., man date: 07/17, exp date: 06/2020) ivy’s (a make of Gilsan manufacturing co. Ltd., man date: 05/18, exp date: 04/18) and normal saline which  showed that Dettol and Izal were effective which aided in the healing process while Ivy and normal saline were effective but slow in the healing process. The probable organisms identified and isolated were species of Staphylococcus and E.coli which are responsible for the infection on wounds of animals. It can be seen from the result that Dettol and Izal are very effective in the treatment of wound.

CHAPTER ONE
INTRODUCTION
Liquid disinfectants are antimicrobial agents that are applied to non-living objects to destroy microorganisms that are living on the objects. The process of killing the microbes is called disinfection which is the killing of some or all of the pathogenic organisms that cause infection. Disinfection does not necessarily kill all microorganisms, especially resistant bacteria spores; it is less effective than sterilization, which is an extreme physical and chemical process that kills all types of life (U.S Center for Disease Control and Prevention, 2016). Disinfectants are different from other antimicrobial agents such as antibiotics, which destroy microorganisms within the body, and antiseptics, which destroy microorganisms on living tissue. Disinfectants are also different from biocides. The latter are intended to destroy all forms of life not just microorganisms. Disinfectants work by destroying the cell wall of microbes or interfering with the metabolism.
Sanitizers are substances that simultaneously clean and disinfect (Mid Sussex District Council 2009). Disinfectants are frequently used in hospitals, dental surgeries, kitchens, and bathrooms to kill infectious organisms. Bacterial endospores are most resistant to disinfectants, but some viruses and bacteria also possess some tolerance.
There are arguments for creating or maintaining conditions that are not conducive to bacterial survival and multiplication, rather than attempting to kill them with chemicals. Bacteria can increase in number very quickly, which enables them to evolve rapidly. Should some bacteria survive a chemical attack, they give rise to new generations composed completely of bacteria that are resistance to particular chemical used. Under a sustained chemical attack, the surviving bacteria in successive generations are increasingly resistant to the chemical used, and ultimately the chemical is rendered ineffective.

1.1       AIM
·         To determine the etopic application of disinfectants on wounds using animal model (Albino rats)

1.2       OBJECTIVES
·         To determine the time duration of healing using animal models
·         To determine the efficacy of the chosen disinfectants

·         To identify the organism isolated from the wounds

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Item Type: Project Material  |  Attribute: 34 pages  |  Chapters: 1-5
Format: MS Word  |  Price: N3,000  |  Delivery: Within 30Mins.
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